Management of Eleusine indica (L.) Gaertn Resistance to Glyphosate Herbicide in Indonesia

Eleusine indica (L.) Gaertn, commonly known as goosegrass or wiregrass, is a type of grass that widespread in many parts the world. The broad-spectrum herbicide glyphosate most frequently used Indonesian oil palm plantations to get rid weeds and other undesirable plants. However, improper rotation types by farmers has led an increased risk resistant weed emergence. This investigation tries validate E. indica’s resistance, investigate mutations EPSPS gene biotype, determine herbicides can control glyphosate-resistant biotypes. whole plant pot test method was measure resistance level, while DNA sequencing using PCR conducted on all samples identify biotype. study results showed biotypes were identified but susceptible propaquizafop, ametryn, sulfentrazone herbicides. Several biotypes, such North Sumatra having multiple resistances glyphosate, paraquat, ammonium glufosinate. Thr102Iso Pro106Ser amino acid substitutions found indica-resistant findings this paraquat glufosinate; further research required mechanism.